Applied Biosystems’ QuantStudio Real-Time PCR systems can detect very small changes, up to 1.5-fold, in gene expression that may be missed by other systems. A dynamic range of up to 10 log tracks large variations in expression across different sample types and conditions. QuantStudio systems include the OptiFlex system, which improves well-to-well and instrument-to-instrument data accuracy. This system enables advanced multiplexing of multiple genes through decoupled excitation and emission filter channels. With the OptiFlex system, TaqMan and SYBR-based assays can be run in the same run.