High-resolution typing of Class I and II HLA alleles.
The AllType FASTplex NGS assay combines the convenience of multiplex PCR with workflow simplicity by eliminating most of the pipetting, purification, and quantification steps required by traditional methods. By pooling amplicons into a single tube within the first hour, libraries can be prepared for sequencing in less than 3.5 hours with minimal hands-on time.
Gene Coverage: HLA-A Full gene, HLA-B Full gene, HLA-C Full gene, HLA-DRB1 Exon 1 + exon 2 – 3′ UTR, HLA-DRB345 Exon 2 – 3’ UTR, HLA-DQB1 Exon 1 + exon 2 – 3′ UTR, HLA-DQA1 Full gene, HLA-DPB1 Exon 1 + exon 2 – 3′ UTR, HLA-DPA1 Full gene